4MU1
The structure of wt A. thaliana IGPD2 in complex with Mn2+, imidazole, and sulfate at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9507 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 113.880, 113.880, 113.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.260 - 1.500 |
| R-factor | 0.15958 |
| Rwork | 0.158 |
| R-free | 0.18832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f1d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.571 |
| Data reduction software | XDS ((XIA2)) |
| Data scaling software | XSCALE ((XIA2)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.676 |
| Number of reflections | 40587 | |
| <I/σ(I)> | 31.8 | 4 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 12.8 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 10% PEG3000, 0.2 M lithium sulfate, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
