4MTT
Ni- and Zn-bound GloA2 at low resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 51.070, 51.070, 327.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.060 - 2.170 |
| R-factor | 0.1771 |
| Rwork | 0.174 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mtr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 2.290 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.173 | 0.733 |
| Number of reflections | 14584 | |
| <I/σ(I)> | 15.2 | 4.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 18.8 | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 31% PEG3350, 0.1 M Tris, 0.2 M magnesium chloride, 0.015 mM CYMAL-7, pH 8.0, VAPOR DIFFUSION, temperature 293K |
