4MTM
Crystal structure of the tail fiber gp53 from Acinetobacter baumannii bacteriophage AP22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9200 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 51.200, 51.200, 302.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.719 - 1.368 |
| R-factor | 0.1009 |
| Rwork | 0.099 |
| R-free | 0.13170 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX (C/D/E) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.990 | 1.440 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.056 | |
| Number of reflections | 62273 | |
| <I/σ(I)> | 33.3 | |
| Completeness [%] | 96.6 | 99.2 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 24% PEG 4000, 0.225M lithium sulphate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
