4MT8

Structure of the ERS1 dimerization and histidine phosphotransfer domain from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-03-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.923
Spacegroup name	C 2 2 21
Unit cell lengths	75.042, 98.747, 77.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 1.900
R-factor	0.1964
Rwork	0.195
R-free	0.22650
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.509
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	PHENIX ((phenix.refine: dev_1092))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	22944
<I/σ(I)>	14.3
Completeness [%]	99.4	97.6
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.18 M l-proline, 0.1 M HEPES pH 7.5, 9% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K