4MT4
Crystal structure of the Campylobacter jejuni CmeC outer membrane channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.827, 146.324, 418.866 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.373 |
R-factor | 0.2114 |
Rwork | 0.210 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.492 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1439) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.460 |
High resolution limit [Å] | 2.370 | 4.930 | 2.370 |
Rmerge | 0.109 | 0.072 | 0.488 |
Number of reflections | 204082 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 93.7 | 93.3 | 86.6 |
Redundancy | 1.8 | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 298 | 30 PEG400, 0.2M NK4SO4, 0.1M NaAC (5.0), VAPOR DIFFUSION, temperature 298K |