4MSW
Y78 ester mutant of KcsA in high K+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Wavelength(s) | 1.00003 |
Spacegroup name | I 4 |
Unit cell lengths | 154.530, 154.530, 75.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.423 - 2.060 |
R-factor | 0.1994 |
Rwork | 0.196 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.050 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.000 | 42.000 | 2.100 |
High resolution limit [Å] | 2.060 | 5.590 | 2.060 |
Rmerge | 0.077 | 0.039 | 0.566 |
Number of reflections | 54941 | ||
<I/σ(I)> | 13.3 | ||
Completeness [%] | 99.8 | 99.2 | 97.2 |
Redundancy | 7.2 | 7.4 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG400, Magnesium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |