4MSP
Crystal structure of human peptidyl-prolyl cis-trans isomerase FKBP22 (aka FKBP14) containing two EF-hand motifs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-22 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.430, 101.200, 58.970 |
| Unit cell angles | 90.00, 100.01, 90.00 |
Refinement procedure
| Resolution | 23.194 - 1.900 |
| R-factor | 0.1597 |
| Rwork | 0.158 |
| R-free | 0.19980 |
| Structure solution method | MAD + MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.055 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.315 |
| Number of reflections | 33131 | |
| <I/σ(I)> | 12.9 | 4.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295 | 25% 1,2-propanediol, 12% PEG 20000, 0.1M MES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
