4MS4
Crystal structure of the extracellular domain of human GABA(B) receptor bound to the agonist baclofen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 115.383, 140.390, 59.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.820 - 1.900 |
R-factor | 0.1912 |
Rwork | 0.190 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mqe |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.073 | 0.025 | 0.678 |
Number of reflections | 75350 | ||
<I/σ(I)> | 10.2 | ||
Completeness [%] | 98.2 | 98.9 | 96.7 |
Redundancy | 7.1 | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 20% PEG2000, 15% glycerol, 0.2 M ammonium chloride, 0.1 M sodium cacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |