4MS2
Structural basis of Ca2+ selectivity of a voltage-gated calcium channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.790, 177.540, 131.140 |
| Unit cell angles | 90.00, 132.69, 90.00 |
Refinement procedure
| Resolution | 40.367 - 2.750 |
| R-factor | 0.2332 |
| Rwork | 0.232 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rvy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.377 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.370 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.078 | |
| Number of reflections | 74174 | |
| Completeness [%] | 96.0 | 96.9 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | 0.1M Na-citrate, pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
