4MRO
Human GKRP bound to AMG-5980 and S6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 65 |
| Unit cell lengths | 149.129, 149.129, 132.383 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.000 - 2.200 |
| R-factor | 0.23263 |
| Rwork | 0.230 |
| R-free | 0.28816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Internal structure |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.879 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 72687 |
| Completeness [%] | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | Well buffer: 0.1M Bis-tris, 0.2M NaI, 16% PEG 8K, pH 7.5, VAPOR DIFFUSION, temperature 298K |
