4MR7
Crystal structure of the extracellular domain of human GABA(B) receptor bound to the antagonist CGP54626
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.670, 113.210, 73.460 |
Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
Resolution | 21.670 - 2.150 |
R-factor | 0.2048 |
Rwork | 0.203 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mqe |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.190 |
High resolution limit [Å] | 2.150 | 5.840 | 2.150 |
Rmerge | 0.042 | 0.024 | 0.393 |
Number of reflections | 57756 | ||
<I/σ(I)> | 14.8 | ||
Completeness [%] | 94.3 | 96.1 | 58.9 |
Redundancy | 3.2 | 3.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10% PEG3350, 20% glycerol, 0.12 M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |