4MP3
Staphyloferrin B precursor biosynthetic enzyme selenomethionine-labeled SbnB
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97649 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.887, 62.887, 157.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.730 - 2.110 |
| Rwork | 0.174 |
| R-free | 0.21390 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.428 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.730 | |
| High resolution limit [Å] | 2.106 | 2.110 |
| Number of reflections | 19092 | |
| Completeness [%] | 99.7 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-tris, 0.2 M LiSO4, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
