4MOZ
Fructose-bisphosphate aldolase from Slackia heliotrinireducens DSM 20476
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 135.783, 174.202, 203.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.1715 |
| Rwork | 0.169 |
| R-free | 0.22320 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.955 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.121 | 0.765 |
| Number of reflections | 128506 | |
| <I/σ(I)> | 18 | 2.2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 90 mM HEPES, 1.26M Sodium Citrate, 10% Glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
