4MOV

1.45 A Resolution Crystal Structure of Protein Phosphatase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	65.724, 77.600, 133.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.929 - 1.450
R-factor	0.1509
Rwork	0.150
R-free	0.16650
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3e7a
RMSD bond length	0.013
RMSD bond angle	1.536
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.929	1.480
High resolution limit [Å]	1.450	1.450
Number of reflections	120264
Completeness [%]	99.4	93.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M HEPES, 1.0 M Lithium Chloride, 20% PEG 6000, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K