4MNT
Crystal structure of human DJ-1 in complex with Cu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.378 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.240, 75.240, 75.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.628 - 1.584 |
| R-factor | 0.1521 |
| Rwork | 0.151 |
| R-free | 0.17740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.117 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.640 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.063 | 0.292 |
| Number of reflections | 33930 | |
| <I/σ(I)> | 7.5 | 2.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 15.5 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Sodium Malonate, 20% w/v PEG 3350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
