4MN9

Fifteen minutes iron loaded frog M ferritin mutant H54Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-08
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	F 4 3 2
Unit cell lengths	183.930, 183.930, 183.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.470 - 1.150
R-factor	0.141
Rwork	0.141
R-free	0.15300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ka3
RMSD bond length	0.004
RMSD bond angle	0.976
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.470	1.210
High resolution limit [Å]	1.150	1.150
Rmerge	0.077	0.404
Number of reflections	93348
<I/σ(I)>	16.7	3.3
Completeness [%]	99.2	95
Redundancy	12.2	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	277	1.6-2M magnesium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1		9	277	1.6-2M magnesium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K