4MMV

Crystal Structure of Prefusion-stabilized RSV F Variant DS-Cav1-TriC at pH 9.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-07-11
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.000
Spacegroup name	P 41 3 2
Unit cell lengths	170.241, 170.241, 170.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.130 - 2.810
R-factor	0.2165
Rwork	0.213
R-free	0.27906
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	21005
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	1.4M sodium potassium tartarate, 0.1M CHES, pH 9.5, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K