4MMR

Crystal Structure of Prefusion-stabilized RSV F Variant Cav1 at pH 9.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-26
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.00
Spacegroup name	P 41 3 2
Unit cell lengths	170.852, 170.852, 170.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.660 - 3.100
R-factor	0.2212
Rwork	0.219
R-free	0.26403
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.599
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Number of reflections	15925
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	1.4M sodium potassium tartarate, 0.1M CHES, pH 9.5, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K