4MMI
Crystal structure of heparan sulfate lyase HepC mutant from Pedobacter heparinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.674, 102.897, 149.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.940 - 2.400 |
| R-factor | 0.20719 |
| Rwork | 0.205 |
| R-free | 0.24907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mmh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 | 0.398 |
| Number of reflections | 27715 | |
| <I/σ(I)> | 38.3 | 4.1 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
