4MM2
Crystal structure of yeast primase catalytic subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.2826 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.370, 196.040, 53.960 |
| Unit cell angles | 90.00, 114.72, 90.00 |
Refinement procedure
| Resolution | 43.880 - 1.600 |
| R-factor | 0.25689 |
| Rwork | 0.255 |
| R-free | 0.29293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mhq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.108 | 1.988 |
| Number of reflections | 111075 | |
| <I/σ(I)> | 10.17 | 0.89 |
| Completeness [%] | 92.6 | 59.8 |
| Redundancy | 5.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 0.1M Na-Citrate, 0.2M NDSB, 5mM CdCl2, 30% PEG 600, 10mM ZnCl2 , pH 5.5, VAPOR DIFFUSION, temperature 298K |
