4MKS

Crystal structure of enolase from Lactobacillus gasseri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	I 4
Unit cell lengths	145.240, 145.240, 99.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.450 - 2.079
R-factor	0.1914
Rwork	0.189
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SWISSMODEL generated using 1w6t template
RMSD bond length	0.003
RMSD bond angle	0.763
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (Autobuild)
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	54.450
High resolution limit [Å]	2.079
Number of reflections	62294

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	297	pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K