4MJQ
E. coli sliding clamp in complex with Bromfenac
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-08-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.993, 65.512, 73.438 |
| Unit cell angles | 73.03, 85.29, 85.65 |
Refinement procedure
| Resolution | 27.980 - 1.730 |
| R-factor | 0.1869 |
| Rwork | 0.185 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.277 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 3.730 | 1.730 |
| Rmerge | 0.034 | 0.025 | 0.218 |
| Number of reflections | 71197 | ||
| <I/σ(I)> | 22.8 | ||
| Completeness [%] | 93.6 | 98.5 | 89.8 |
| Redundancy | 3.9 | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 100mM MES, 100-150mM CaCl2, 25-30%(v/v) PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
