4MJO
Human liver fructose-1,6-bisphosphatase(d-fructose-1,6-bisphosphate, 1-phosphohydrolase) (e.c.3.1.3.11) complexed with the allosteric inhibitor 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | CCD |
| Collection date | 2007-03-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.974, 286.305, 83.692 |
| Unit cell angles | 90.00, 97.79, 90.00 |
Refinement procedure
| Resolution | 29.740 - 2.400 |
| R-factor | 0.20817 |
| Rwork | 0.207 |
| R-free | 0.23647 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.521 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.500 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 115242 |
| Completeness [%] | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 0.1 M ammonium acetate and 12% polyethylenglycol 3350 in 0.1 M HEPES, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
