4MJI
T cell response to a HIV reverse transcriptase epitope presented by the protective allele HLA-B*51:01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.510, 88.870, 129.270 |
| Unit cell angles | 102.91, 95.92, 90.09 |
Refinement procedure
| Resolution | 64.750 - 2.990 |
| R-factor | 0.2448 |
| Rwork | 0.242 |
| R-free | 0.30380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.713 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.753 | 64.753 | 3.070 |
| High resolution limit [Å] | 2.990 | 13.370 | 2.990 |
| Rmerge | 0.090 | 0.363 | |
| Rmeas | 0.097 | 0.424 | |
| Rpim | 0.065 | 0.284 | |
| Total number of observations | 891 | 5907 | |
| Number of reflections | 39174 | ||
| <I/σ(I)> | 4.8 | 6.9 | 2.5 |
| Completeness [%] | 98.6 | 96.5 | 98.1 |
| Redundancy | 2 | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 20% PEG 3350, 0.2M NaSO4, 0.1M Bis-Tris Propane, pH 6.5, VAPOR DIFFUSION, temperature 291K |
