4MIH
Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.04094 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.100, 167.099, 168.472 |
| Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
| Resolution | 48.558 - 2.400 |
| R-factor | 0.2058 |
| Rwork | 0.205 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 233009 | |
| <I/σ(I)> | 11.9 | 1.7 |
| Completeness [%] | 98.4 | 97.4 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M HEPES, 0.2 M NH4Cl, 15% (w/v) PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
