4MHJ
Crystal structure of Fab H5M9 in complex with influenza virus hemagglutinin from A/goose/Guangdong/1/96 (H5N1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 199.552, 199.552, 466.886 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.080 - 6.980 |
| R-factor | 0.38 |
| Rwork | 0.377 |
| R-free | 0.38700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3GBM AND 4MHI |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 174.078 | 7.440 |
| High resolution limit [Å] | 6.979 | 6.979 |
| Number of reflections | 16538 | |
| <I/σ(I)> | 12.9 | 1.2 |
| Completeness [%] | 93.3 | 64 |
| Redundancy | 5.8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M HEPES, pH 7.5, 2% PEG400, 2.0 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
