4MH4

Crystal Structure of OsmC-like protein from Burkholderia cenocepacia J2315

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 43
Unit cell lengths	87.940, 87.940, 44.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 1.900
R-factor	0.167
Rwork	0.166
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zb8
RMSD bond length	0.012
RMSD bond angle	1.493
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.037	0.018	0.419
Number of reflections	27321	319	2016
<I/σ(I)>	30.53	76.65	4.32
Completeness [%]	99.7	92.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	289	JCSG+(c1): 20%PEG-8000, 100mM Sodium Phosphate Dibasic/ Citric Acid 200mM Sodium Chloride, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K