4MH3
Crystal structure of Bovine Mitochondrial Peroxiredoxin III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 142.895, 290.885, 81.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 102.140 - 2.400 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zye |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.581 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 288.670 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.732 |
| Number of reflections | 130007 | |
| <I/σ(I)> | 11.3 | 1.8 |
| Completeness [%] | 97.7 | 93.9 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 289 | containing 36% 2-Methyl-1,3-Propanediol, 0.1M phosphate-citrate, pH 4.1, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
