4MGN

Co-crystal structure of the G. kaustophilus glyQS T box riboswitch Stem I in complex with tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	92.838, 205.182, 161.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.292 - 3.200
R-factor	0.2406
Rwork	0.236
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4JRC and 4MGM
RMSD bond length	0.001
RMSD bond angle	0.458
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.160	0.050
Number of reflections	26207
<I/σ(I)>	4.2	56.78	2.29
Completeness [%]	98.8	97.7	96.9
Redundancy	6.9	7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7	291	80mM NaCl, 20mM MgCl2, 40mM sodium cacodylate, 12mM spermine, 10% 2-methyl-1,3 propanediol, pH 7.0, hanging drop, temperature 291K