4MFU

Crystal structure of human CTNNBL1(residues 77~563)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97985
Spacegroup name	P 31 2 1
Unit cell lengths	90.149, 90.149, 178.557
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.330 - 2.744
R-factor	0.2507
Rwork	0.247
R-free	0.31870
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.426
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.380	50.000	2.840
High resolution limit [Å]	2.740	5.900	2.740
Rmerge	0.095	0.080	0.328
Number of reflections	22673
<I/σ(I)>	36.9	112.2	2.2
Completeness [%]	95.9	98.4	71.5
Redundancy	13.9	19.2	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	295	PEG8000, CAPs, NaCl, pH 9.5, vapor diffusion, hanging drop, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	9.5	295	PEG8000, CAPs, NaCl, pH 9.5, vapor diffusion, hanging drop, temperature 295K