4MF3
Crystal Structure of Human GRIK1 complexed with a 6-(tetrazolyl)aryl decahydroisoquinoline antagonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.018, 108.018, 109.766 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.2381 |
| Rwork | 0.237 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.650 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Number of reflections | 24693 | |
| Completeness [%] | 98.2 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | Protein mixed 1:1 with 55% MPD, 0.2M NH4H2PO4, 0.1M TRIS pH 8.0, VAPOR DIFFUSION, temperature 293K |
