4MEM

Crystal Structure of the rat USP11 DUSP-UBL domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.973
Spacegroup name	P 2 21 21
Unit cell lengths	37.285, 80.156, 149.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.622 - 2.340
R-factor	0.2228
Rwork	0.221
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mel
RMSD bond length	0.004
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	74.635
High resolution limit [Å]	2.292
Number of reflections	20914
Completeness [%]	99.7
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.77	292	0.15 M Malic Acid, 24% w/v PEG 3350, 10 mM Strontium Chloride, pH 6.77, VAPOR DIFFUSION, SITTING DROP, temperature 292K