4ME7
Crystal structure of Bacillus subtilis toxin MazF in complex with cognate antitoxin MazE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.503, 91.618, 104.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.330 - 2.918 |
| R-factor | 0.2574 |
| Rwork | 0.253 |
| R-free | 0.29890 |
| Structure solution method | SAD |
| Starting model (for MR) | 4mdx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.330 | 3.000 |
| High resolution limit [Å] | 2.918 | 2.918 |
| Rmerge | 0.086 | 0.668 |
| Number of reflections | 17226 | |
| <I/σ(I)> | 31.6 | 1.85 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M calcium chloride, 0.1 M Bis-Tris pH 6.5 and 3% (v/v) isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
