4MDW

Crystal structure of a DUF1541 family protein (ydhK) from Bacillus subtilis subsp. subtilis str. 168 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97926,0.97885
Spacegroup name	P 21 21 21
Unit cell lengths	27.692, 64.174, 80.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.692 - 2.000
R-factor	0.1824
Rwork	0.179
R-free	0.25970
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.692		2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.090	0.030	0.692
Number of reflections	9897	1793	1527
<I/σ(I)>	7.1	21.1	1.4
Completeness [%]	94.7	94.1	83.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	5.0% polyethylene glycol 3000, 40.0% polyethylene glycol 400, 0.1M MES pH 6.0, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 277K