4MD7

Crystal Structure of full-length symmetric CK2 holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 1 21 1
Unit cell lengths	142.316, 57.958, 186.188
Unit cell angles	90.00, 102.42, 90.00

Refinement procedure

Resolution	181.832 - 3.100
R-factor	0.2347
Rwork	0.233
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4DGL AND 4KWP
RMSD bond length	0.007
RMSD bond angle	1.126
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	181.832	48.872	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge		0.050	0.643
Total number of observations		8421	40507
Number of reflections	52811
<I/σ(I)>	10.2	12	1.2
Completeness [%]	97.9	91.1	98.7
Redundancy	5.3	5.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	20% PEG3350, 0.2 M ammonium citrate, pH 6.5, VAPOR DIFFUSION, temperature 277K