4MD7
Crystal Structure of full-length symmetric CK2 holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 142.316, 57.958, 186.188 |
| Unit cell angles | 90.00, 102.42, 90.00 |
Refinement procedure
| Resolution | 181.832 - 3.100 |
| R-factor | 0.2347 |
| Rwork | 0.233 |
| R-free | 0.26330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4DGL AND 4KWP |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 181.832 | 48.872 | 3.270 |
| High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
| Rmerge | 0.050 | 0.643 | |
| Total number of observations | 8421 | 40507 | |
| Number of reflections | 52811 | ||
| <I/σ(I)> | 10.2 | 12 | 1.2 |
| Completeness [%] | 97.9 | 91.1 | 98.7 |
| Redundancy | 5.3 | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 20% PEG3350, 0.2 M ammonium citrate, pH 6.5, VAPOR DIFFUSION, temperature 277K |
