4MD2
Ground state of bacteriorhodopsin from Halobacterium salinarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 63 |
Unit cell lengths | 60.910, 60.910, 110.090 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.440 - 1.730 |
R-factor | 0.142 |
Rwork | 0.139 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.440 | 1.820 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.049 | 0.342 |
Number of reflections | 22537 | |
<I/σ(I)> | 12.7 | 2.7 |
Completeness [%] | 93.9 | 95.3 |
Redundancy | 2.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | in meso | 5.6 | 295 | Crystals were obtained in a lipidic cubic phase of monoolein, pH 5.6, in meso, temperature 295K |