4MCK
Crystal structure of Family GH19, Class IV chitinase from Zea mays
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-08-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.152, 65.167, 72.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.915 - 1.500 |
R-factor | 0.1802 |
Rwork | 0.178 |
R-free | 0.20310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hbd |
RMSD bond length | 0.007 |
RMSD bond angle | 1.248 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.416 | |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.044 | |
Number of reflections | 28893 | |
<I/σ(I)> | 3.3 | |
Completeness [%] | 96.7 | 63.2 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.5 M ammonium sulfate, 0.1 M Tris, 0.16 M potassium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |