4MBX
Structure of unliganded B-Lymphotropic Polyomavirus VP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2012-07-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.010, 95.920, 231.680 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 39.380 - 1.920 |
| R-factor | 0.16655 |
| Rwork | 0.165 |
| R-free | 0.19813 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vpn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 246146 | |
| <I/σ(I)> | 10.5 | 1.67 |
| Completeness [%] | 98.8 | 99.5 |
| Redundancy | 2.97 | 3.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 12% (v/v) Isopropanol, 0.2 M calcium chloride, 0.1 M sodium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
