4MAT

E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1997-10-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	38.648, 68.298, 55.449
Unit cell angles	90.00, 105.98, 90.00

Refinement procedure

Resolution	28.800 - 2.000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2mat
RMSD bond length	0.012
RMSD bond angle	17.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	TNT (5F PRERELEASE)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.047 *	0.210 *
Total number of observations	48052 *
Number of reflections	16062
<I/σ(I)>	22.4	4.4
Completeness [%]	85.2	70.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.7 (mg/ml)
2	1	drop	dithiothreitol	20 (mM)
3	1	reservoir	PEG3400	22-27 (%)
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir		200 (mM)