4MAH
Structure of Aspergillus oryzae AA11 Lytic Polysaccharide Monooxygenase with Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.282 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.207, 61.498, 65.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.070 - 1.550 |
| R-factor | 0.1337 |
| Rwork | 0.132 |
| R-free | 0.16560 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.420 |
| Data reduction software | XDS |
| Data scaling software | SCALA (0.1.29) |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.070 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.052 | 0.370 |
| Number of reflections | 32367 | |
| <I/σ(I)> | 17.7 | 3.6 |
| Completeness [%] | 98.7 | 94.2 |
| Redundancy | 5.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 292 | 10 mM zinc chloride, 100 mM MES, pH 6.0, 20% w/v PEG6000, VAPOR DIFFUSION, temperature 292K |
