4MA7
Crystal structure of mouse prion protein complexed with Promazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.211, 106.038, 75.733 |
| Unit cell angles | 90.00, 95.68, 90.00 |
Refinement procedure
| Resolution | 34.880 - 1.970 |
| R-factor | 0.21741 |
| Rwork | 0.216 |
| R-free | 0.24687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h88 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 52155 | |
| <I/σ(I)> | 15.4 | 2 |
| Completeness [%] | 98.3 | 85.1 |
| Redundancy | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG3350, 0.1 M Bis-Tris, pH 6.5, 0.2 M lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
