4MA4
S-glutathionylated PFKFB3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 102.508, 102.508, 260.606 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.700 - 2.230 |
| R-factor | 0.20154 |
| Rwork | 0.199 |
| R-free | 0.24181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.180 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.680 | |
| Number of reflections | 37905 | |
| Completeness [%] | 99.7 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 285 | 50 mM MES, pH 6.0, 7% (w/v) polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
