4M9V
Zfp57 mutant (E182Q) in complex with 5-carboxylcytosine DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.388, 96.093, 36.408 |
| Unit cell angles | 90.00, 113.14, 90.00 |
Refinement procedure
| Resolution | 33.459 - 0.969 |
| R-factor | 0.1307 |
| Rwork | 0.130 |
| R-free | 0.14260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gzn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 1.000 |
| High resolution limit [Å] | 0.969 | 0.969 |
| Rmerge | 0.070 | 0.648 |
| Number of reflections | 129957 | |
| <I/σ(I)> | 11.7 | 1.4 |
| Completeness [%] | 95.7 | 92.7 |
| Redundancy | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 35% 2-methyl-2,4-pentanediol, 15% polyethylene glycol 8000, 100 mM CaCl2, and 100 mM acetate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
