4M9U
The crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit of Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 85.313, 85.313, 107.673 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.962 - 1.599 |
R-factor | 0.1676 |
Rwork | 0.166 |
R-free | 0.19550 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.087 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 1.630 |
High resolution limit [Å] | 1.599 | 1.600 |
Rmerge | 0.079 | 0.705 |
Number of reflections | 53157 | |
<I/σ(I)> | 44.5 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.5 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.8M Sodium Acetate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |