4M9P

Crystal structure of the human filamin A Ig-like domains 3-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976250
Spacegroup name	P 65
Unit cell lengths	63.041, 63.041, 136.604
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.650 - 1.720
R-factor	0.201
Rwork	0.199
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2NQC AND 3V8O CHAIN A
RMSD bond length	0.019
RMSD bond angle	1.967
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.1)
Refinement software	REFMAC (refmac_5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.650	1.760
High resolution limit [Å]	1.720	1.720
Rmerge	0.093	0.635
Number of reflections	32462
<I/σ(I)>	18.24	3.3
Completeness [%]	99.7	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.1 M sodium malonate, 12% w/v PEG3350, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K