4M9J
DNA Polymerase Beta E295K Soaked with dUMPNPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 104 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.580, 79.129, 54.979 |
| Unit cell angles | 90.00, 105.95, 90.00 |
Refinement procedure
| Resolution | 13.984 - 2.038 |
| R-factor | 0.1909 |
| Rwork | 0.186 |
| R-free | 0.22990 |
| Structure solution method | Isomorphous replacement |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.707 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 14.000 | 14.000 | 2.110 |
| High resolution limit [Å] | 2.038 | 4.350 | 2.040 |
| Rmerge | 0.089 | 0.044 | 0.593 |
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.2 | 100 | 92.4 |
| Redundancy | 6.9 | 7.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 14% PEG 3350, 300 mM sodium acetate pH 9, 50 mM HEPES pH 7.5, vapor diffusion, hanging drop, temperature 291K |
