4M94
d(ATCCGTTATAACGGAT) complexed with Moloney Murine Leukemia virus reverse transcriptase catalytic fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-27 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.957, 145.722, 46.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.730 - 2.140 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.070 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.730 |
High resolution limit [Å] | 2.140 |
Rmerge | 0.047 |
Number of reflections | 19858 |
<I/σ(I)> | 21.7 |
Completeness [%] | 93.7 |
Redundancy | 3.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 7% PEG 4000, 5mM Magnesium acetate, 50mM N-(2-acetamido)iminodiacetic acid, pH 6.5, vapor diffusion, hanging drop, temperature 293.15K |