4M8L
crystal structure of RpiA-R5P complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.203, 151.958, 36.290 |
| Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
| Resolution | 36.750 - 2.370 |
| R-factor | 0.2394 |
| Rwork | 0.237 |
| R-free | 0.27434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kwm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.999 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.370 | 2.400 |
| Rmerge | 0.090 | 0.328 |
| Number of reflections | 34431 | |
| <I/σ(I)> | 13.354 | 3.709 |
| Completeness [%] | 94.5 | 99.9 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 0.22 M magnesium chloride 0.1M Tris pH 6.5, 21% (w/v) PEG 4000, additive: 40% formamide, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
