4M8I
1.43 Angstrom resolution crystal structure of cell division protein FtsZ (ftsZ) from Staphylococcus epidermidis RP62A in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.335, 52.063, 87.005 |
| Unit cell angles | 90.00, 109.64, 90.00 |
Refinement procedure
| Resolution | 27.330 - 1.430 |
| R-factor | 0.17061 |
| Rwork | 0.169 |
| R-free | 0.20616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vo8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.055 | 0.548 |
| Number of reflections | 53059 | |
| <I/σ(I)> | 23.42 | 2.45 |
| Completeness [%] | 95.8 | 91.4 |
| Redundancy | 5.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 6.7 mg/mL protein in 10 mM Tris-HCl, pH 8.3, 0.25 M sodium chloride, 5 mM BME, crystallization conditions: Classics II G5 (#77): 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, 25% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
