4M80

The structure of E292S glycosynthase variant of exo-1,3-beta-glucanase from Candida albicans at 1.85A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2004-08-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.985, 65.331, 96.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.054 - 1.858
R-factor	0.1409
Rwork	0.139
R-free	0.17640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cz1
RMSD bond length	0.007
RMSD bond angle	1.059
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.240	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.080	0.364
Number of reflections	32719
<I/σ(I)>	12.3	4.1
Completeness [%]	98.4	89.2
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	291	0.1M HEPES-KOH, 0.2M CACL2, 19% PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K